Colloidal Magnetic Silica Nano-/Microspheres
 

  • CV< 15 % for Nanospheres below 1 μm and CV< 5% for Microspheres above 1 μm

  • Roundness > 0.980

  • Density: ~2.5 g/cm^3

  • Saturation magnetization: ~20 emu/g

  • Provided in Milli-Q water

  • Superparamagnetic cores covered by the silica shells

  • Surface functional group: -OH available for all sizes and -NH2 and -COOH available for 1 μm

  • Low concentrations to ultra-high concentration available: 10 - 100 mg/ml (i.e. 1 - 10 % w/v)

  • Sizes available: 100 nm, 300 nm, 500 nm, 1 μm, 2 μm, 5 μm, 10 μm, and 50 μm

  • Although applications vary from one to another, 1-10 mg solid magnetic silica microspheres will typically yield 10 μg of DNA molecules 

  • Lifetime: At least 2 years from the date of product delivery

  • These iron oxide beads coated with silica can be easily surface functionalized via chemical bond formation. The non-functionalized magnetic silica nanoparticles (1 μm being the most popular size) are frequently used for plasmid DNA/RNA isolation/extraction. Carboxyl-functionalized magnetic silica nanoparticles and amine-terminated magnetic silica beads are known to be effective in protein isolation or macromolecule purification.

  • Can be adapted to other common biological buffers like phosphate-buffered saline (PBS) or organic solvents like ethanol via repeated centrifugation and resuspension.

  • The magnetic silica microspheres might precipitate at the bottom of the containing vial over time, and this is not a sign of aggregation. Large particles precipitate faster than small particles. These particles can be easily resuspended using sonication (ultrasonic bath or ultrasonic homogenizer) or vortexing.

  • Our magnetic silica beads are only priced based on the solid content; for instance, 50 ml of the silica nanospheres at 10mg/ml would have the same price as 10 ml of the silica nanospheres at 50 mg/ml.

Safety Data Sheets

Magnetic Responsiveness​

Silica Magnetic Microspheres
Silica Magnetic Microspheres

Fully suspended microspheres when they are away from the magnetic field

Aggregated microspheres at the poles when they are in the magnetic field

magnetic silica microspheres

Our colloidal magnetic silica beads & microspheres respond to the magnetic field, enabling many laboratory and commercial applications. These microspheres can be repetitively used and easily coupled with other surface moieties for further functionalizations. 

Scanning/Transmission Electron Micrographs

100 nm

Alpha Nanotech Magnetic Silica Nanoparticles
Alpha Nanotech Magnetic Silica Nanoparticles

300 nm

Alpha Nanotech Magnetic Silica Nanoparticles
Alpha Nanotech Magnetic Silica Nanoparticles

1 μm

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2 μm

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5 μm

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10 μm

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50 μm

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Zeta Potential Measurements at pH 7 for Hydroxyl-, Carboxyl-, and Amine-Terminated Magnetic Silica Beads & Microspheres

Hydroxyl-Terminated Magnetic Silica Microspheres

Carboxyl-Terminated Magnetic Silica Microspheres

Amine-Terminated Magnetic Silica Microspheres

 Zeta Potential Measurements at Different pH for Hydroxyl-Terminated Magnetic Silica Beads & Microspheres

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Fourier-transform infrared spectroscopy (FTIR) measurement results

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How do we measure the CV Values and reveal the real size distributions?

Through the years of working with nano-/microspheres, our researchers and technicians fully understand the importance of the size distributions from batch to batch. In the field of size characterizations, there are mainly two methods, scanning/transmission/scanning transmission electron micrographs (SEM/TEM/STEM) and dynamic light scattering (i.e. DLS). Unfortunately, we found DLS are unsuitable for accurately determine the size distributions of nano-/microspheres when compared to SEM/TEM/STEM techniques. 
 

  • DLS results are closely related to colloidal stability, and as an inevitable result, the size distributions will not be accurate for large-sized nanoparticles. For nanoparticles below 100 nm, the hydrodynamic sizes become relevant. However, for nanoparticles around or above a few hundred nanometers, colloidal stability is greatly affected by the role of gravity. In comparison, the SEM/TEM/STEM images are based on dry samples only where higher reproducibility and reliability have been seen through numerous studies in our case and in the literature.

  • DLS cannot distinguish between nanoparticles themselves and the aggregates of surfactants. As most of our colloidal nano-/microspheres have a trace of surfactants purposely added in the system for longer lifetime, DLS becomes unsuitable for direct size measurements unless the nano-/microspheres are washed at least three times with the Milli-Q water or de-ionized water to fully remove any trace of surfactants. Similarly, the nano-/microspheres due to inefficient re-suspension also distort the DLS results; for instance, a couple of aggregated 100 nm nanoparticles might be seen as 200 nm or 300 nm nanoparticles under DLS measurements.

  • DLS cannot give any insight into the shape or the spherical degree of nano-/microspheres. Irregularly-shaped particles and perfectly spherical particles can be interpreted the same under DLS measurements.

 

As a result, we use SEM/TEM/STEM techniques instead of DLS measurements as our routine quality control method. We routinely take at least 10 electron micrographs and process them with ImageJ for size distributions as well as the CV value. This is also the reason that we typically include a couple of representative SEM/TEM/STEM images with our products when delivered to our clients. We wish to reveal the real size distributions and let the clients visualize the images of nano-/microspheres directly instead of viewing the fitted curves indirectly. 

nanoparticle size measurement

Our Colloidal Magnetic Silica Nano-/Microspheres in Stock:

100 nm, Hydroxyl-Terminated
 

300 nm, Hydroxyl-Terminated
 

1 μm, Hydroxyl-Terminated

1 μm, Carboxyl-Terminated

1 μm, Amine-Terminated

μm, Hydroxyl-Terminated

μm, Hydroxyl-Terminated

10 μm, Hydroxyl-Terminated

50 μm, Hydroxyl-Terminated

10 ml at 10 mg/ml

$ 180

$ 180

$ 180

$ 210

$ 210

$ 180

 

$ 180

 

$ 180

 

$ 180

20 ml at 10 mg/ml

$ 290

$ 290

$ 290

$ 360

$ 360

$ 290

 

$ 290

 

$ 290

 

$ 290

10 ml at 50 mg/ml

$ 550

$ 550

 

$ 550

 

$ 610

 

$ 610

 

$ 550

$ 550

$ 550

$ 550

20 ml at 50 mg/ml

$ 890

$ 890

$ 890

$ 1,015

$ 1,015

$ 890

 

$ 890

 

$ 890

 

$ 890

Notes:
 

1. SDS (Material Safety Data Sheet) and COA (Certificate of Analysis) are included for every purchase. 

2. The prices listed above are in Canadian Dollars (CND$), and please refer to the blow exchange box for the most current daily exchange rates.

3. We accept both online purchases at our online store or Amazon stores and invoiced orders (Net 30 Terms). In-stock items will be dispatched within 1-3 business days. The lead time for customized orders is typically within 10 business days and will be evaluated on a case-by-case basis.

 

4. Shipping Locations for our clients are listed below.
 

Orders destined for the United States will be shipped from one of the two US offices below.

Unit 390, 701 Harrison Ave, Blaine, WA 98230 US

Unit 4, 2045 Niagara Falls Blvd, Niagara Falls, NY 14304 US
 

Orders destined for the UK will be shipped from our UK office.

Office 24743, Courier Point, 13 Freeland Park, Wareham Road, Poole, Dorset, BH16 6FH, UK


Orders destined for Europe will be shipped from our EU office.
Allée Primavera 78, Annecy, Pringy, 74370, France


Orders destined for Canada and other International destinations will be shipped from our Canadian headquarters.
135 - 3800 Wesbrook Mall, University of British Columbia Campus, Vancouver, BC, V6S2L9, Canada


No duties or any additional taxes for the orders delivered to Canadian, US, UK, and EU addresses, regardless of the sales channel. For other International shipping addresses, recipients are responsible for local duties and taxes.

 

5. Free exchanges or full refunds will be offered within 30 days after the order fulfillment without any questions asked if the ordered items fail to meet customer expectations.

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